Hotspot

rpl32-trnL-UAG

SSC · 112946-113541 · 596 bp

Back to Brassica

Pi 0.021

MarkerSeek score 55.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 596
variable_sites: 48
pi: 0.021
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTCTACAGGTAATTCAAAAAGT	TCCTAAGAGCAGCGTGTCTA	29.2	50.0	53.5	57.9	683-778 bp	1.0	47.1
rpl32-trnL-UAG_p2	CTTTCTACAGGTAATTCAAAAAGT	AGCAGCGTGTCTACCAATTT	29.2	45.0	53.5	57.8	676-771 bp	1.0	47.1
rpl32-trnL-UAG_p3	CTTTCTACAGGTAATTCAAAAAGT	CCTAAGAGCAGCGTGTCTAC	29.2	55.0	53.5	57.8	682-777 bp	1.0	47.1
rpl32-trnL-UAG_p4	CTTTCTACAGGTAATTCAAAAAGT	CTAAGAGCAGCGTGTCTACC	29.2	55.0	53.5	57.8	681-776 bp	1.0	47.1
rpl32-trnL-UAG_p5	CTTTCTACAGGTAATTCAAAAAGT	GAGCAGCGTGTCTACCAATT	29.2	50.0	53.5	58.3	677-772 bp	1.0	47.1