Hotspot

ndhF-rpl32

SSC · 111958-112786 · 829 bp

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Pi 0.0159

MarkerSeek score 53.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 829
variable_sites: 49
pi: 0.0159
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	47.6	40.0	57.6	55.5	905-929 bp	1.0	56.2
ndhF-rpl32_p2	TGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGA	45.0	40.0	56.3	55.5	904-928 bp	1.0	54.4
ndhF-rpl32_p3	CTGGAAGTGGAATGAAAGGGA	ACGAATACGCTTTTTCGAGAT	47.6	38.1	57.6	56.1	905-929 bp	1.0	52.7
ndhF-rpl32_p4	CTGGAAGTGGAATGAAAGGG	ACGAATACGCTTTTTCGAGA	50.0	40.0	56.0	55.5	905-929 bp	1.0	51.8
ndhF-rpl32_p5	CTGGAAGTGGAATGAAAGGGAT	ACGAATACGCTTTTTCGAGA	45.5	40.0	58.0	55.5	905-929 bp	1.0	51.5