Hotspot

petA-psbJ

LSC · 61793-62781 · 989 bp

Back to Brassica

Pi 0.0133

MarkerSeek score 47.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 50
pi: 0.0133
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAAATCTTTTTGGTTCT	GCTGGTATTCTTGTGATCGGT	36.4	47.6	57.2	58.1	1129-1174 bp	1.0	55.2
petA-psbJ_p2	TGGCACAAATCTTTTTGGTTCT	AGCTGGTATTCTTGTGATCGG	36.4	47.6	57.2	57.8	1130-1175 bp	1.0	54.5
petA-psbJ_p3	TGGCACAAATCTTTTTGGTTCTT	GCTGGTATTCTTGTGATCGGT	34.8	47.6	57.9	58.1	1129-1174 bp	1.0	52.5
petA-psbJ_p4	TGGCACAAATCTTTTTGGTTCTT	AGCTGGTATTCTTGTGATCGG	34.8	47.6	57.9	57.8	1130-1175 bp	1.0	51.7
petA-psbJ_p5	TGGCACAAATCTTTTTGGTTC	GCTGGTATTCTTGTGATCGGT	38.1	47.6	55.8	58.1	1129-1174 bp	1.0	51.4