Hotspot

cemA-petA

LSC · 60605-60829 · 225 bp

Back to Brassica

Pi 0.0142

MarkerSeek score 47.50

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 11
pi: 0.0142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	CGTGTATCTCCGTCACTTGT	GGAGCGAGTAATCTCTTCCC	50.0	55.0	57.4	57.2	320-325 bp	1.0	73.0
cemA-petA_p2	CGTGTATCTCCGTCACTTGT	ATGCACTTGAAATGGATGCC	50.0	45.0	57.4	57.0	375-380 bp	1.0	71.0
cemA-petA_p3	CGTGTATCTCCGTCACTTGT	AGTGAGACAGATATGGAGCGA	50.0	47.6	57.4	58.1	334-339 bp	1.0	70.7
cemA-petA_p4	CGTGTATCTCCGTCACTTGT	TGAGACAGATATGGAGCGAGT	50.0	47.6	57.4	58.1	332-337 bp	1.0	70.7
cemA-petA_p5	TCGTGTATCTCCGTCACTTG	GGAGCGAGTAATCTCTTCCC	50.0	55.0	57.1	57.2	321-326 bp	1.0	70.7