Hotspot

ycf4-cemA

LSC · 59389-59914 · 526 bp

Back to Brassica

Pi 0.0111

MarkerSeek score 48.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 526
variable_sites: 22
pi: 0.0111
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCTTACTTCTTGCGTGT	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.0	58.3	582-639 bp	1.0	84.0
ycf4-cemA_p2	AGGTCAAGGGGCTATTCCTT	AGAGAGATCAACCAGGGCAA	50.0	50.0	58.0	58.3	658-715 bp	1.0	83.8
ycf4-cemA_p3	TTGGCTTACTTCTTGCGTGT	TCAACCAGGGCAAAAAGACT	45.0	45.0	58.0	57.5	575-632 bp	1.0	83.1
ycf4-cemA_p4	AGGTCAAGGGGCTATTCCTT	TCAACCAGGGCAAAAAGACT	50.0	45.0	58.0	57.5	651-708 bp	1.0	82.9
ycf4-cemA_p5	AGGTCAAGGGGCTATTCCTT	GAGAGATCAACCAGGGCAAA	50.0	50.0	58.0	57.5	657-714 bp	1.0	82.6