Hotspot

trnF-GAA-ndhJ

LSC · 47135-47723 · 589 bp

Back to Brassica

Pi 0.0114

MarkerSeek score 51.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 26
pi: 0.0114
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	700-788 bp	1.0	84.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	699-787 bp	1.0	84.9
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	697-785 bp	1.0	83.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	703-791 bp	1.0	82.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CTTATGATAGCCATCCGCGG	50.0	55.0	58.1	58.4	738-826 bp	1.0	82.1