Hotspot

trnS-GCU-trnG-UCC

LSC · 7685-8348 · 664 bp

Back to Brassica

Pi 0.0186

MarkerSeek score 58.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 46
pi: 0.0186
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTT	50.0	45.0	57.8	56.5	750-789 bp	1.0	75.1
trnS-GCU-trnG-UCC_p2	CCAACGCTTTAGTCCACTCA	ATCAAACCGAGAGACCCTTT	50.0	45.0	57.8	56.5	749-788 bp	1.0	75.1
trnS-GCU-trnG-UCC_p3	AACGCTTTAGTCCACTCAGC	AATCAAACCGAGAGACCCTT	50.0	45.0	58.2	56.5	748-787 bp	1.0	74.8
trnS-GCU-trnG-UCC_p4	AACGCTTTAGTCCACTCAGC	ATCAAACCGAGAGACCCTTT	50.0	45.0	58.2	56.5	747-786 bp	1.0	74.8
trnS-GCU-trnG-UCC_p5	CCAACGCTTTAGTCCACTCA	AATCAAACCGAGAGACCCTTT	50.0	42.9	57.8	57.2	750-789 bp	1.0	73.2