Hotspot

rps16-trnQ-UUG

LSC · 6031-6486 · 456 bp

Back to Brassica

Pi 0.0201

MarkerSeek score 55.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 30
pi: 0.0201
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	529-534 bp	1.0	86.6
rps16-trnQ-UUG_p2	AAGGTGGCGGATCAAAACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	575-580 bp	1.0	86.4
rps16-trnQ-UUG_p3	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	539-544 bp	1.0	85.7
rps16-trnQ-UUG_p4	AAAGGTGGCGGATCAAAACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	576-581 bp	1.0	84.6
rps16-trnQ-UUG_p5	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	555-560 bp	1.0	84.0