Hotspot

matK-trnK-UUU

LSC · 3514-4192 · 679 bp

Back to Brassica

Pi 0.014

MarkerSeek score 46.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 41
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCTTGAGTTCTGAAATGACACA	ACGGTAGAGTACTCGGCTTT	36.4	50.0	55.6	58.2	721-725 bp	1.0	49.9
matK-trnK-UUU_p2	TCTTGAGTTCTGAAATGACACA	AACGGTAGAGTACTCGGCTT	36.4	50.0	55.6	58.2	722-726 bp	1.0	49.8
matK-trnK-UUU_p3	TTCTTGAGTTCTGAAATGACACA	AACGGTAGAGTACTCGGCTT	34.8	50.0	56.3	58.2	723-727 bp	1.0	47.6
matK-trnK-UUU_p4	TTCTTGAGTTCTGAAATGACACA	ACGGTAGAGTACTCGGCTTT	34.8	50.0	56.3	58.2	722-726 bp	1.0	47.6
matK-trnK-UUU_p5	TCTTGAGTTCTGAAATGACACA	GGGTTGCTAACTCAACGGTA	36.4	50.0	55.6	57.5	735-739 bp	1.0	47.5