Hotspot

ycf1

SSC · 126449-132070 · 5622 bp

Back to Brassaiopsis

Pi 0.0134

MarkerSeek score 49.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5622
variable_sites: 110
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTTGAGAGAACGGATCCGC	ACCGTGGATTATACCAAGCA	50.0	45.0	58.1	56.6	232-234 bp	1.0	77.5
ycf1_p2	ATCCGCGATTTCCTTGGTTT	ACCGTGGATTATACCAAGCA	45.0	45.0	58.2	56.6	218-220 bp	1.0	76.7
ycf1_p3	CCGCGATTTCCTTGGTTTTG	ACCGTGGATTATACCAAGCA	50.0	45.0	58.3	56.6	216-218 bp	1.0	75.6
ycf1_p4	ATTTGAGAGAACGGATCCGC	AACCGTGGATTATACCAAGCA	50.0	42.9	58.1	57.4	233-235 bp	1.0	75.5
ycf1_p5	ATTTGAGAGAACGGATCCGC	ACCGTGGATTATACCAAGCAA	50.0	42.9	58.1	57.4	232-234 bp	1.0	75.5