Hotspot

ndhF

SSC · 112465-114711 · 2247 bp

Back to Brassaiopsis

Pi 0.00979

MarkerSeek score 49.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 33
pi: 0.00979
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GATGTCCTTGCCCACACTAG	CGAACAGGTTGGTCATCTCA	55.0	50.0	58.0	57.5	2639-2659 bp	1.0	77.9
ndhF_p2	GATGTCCTTGCCCACACTAG	ACGAACAGGTTGGTCATCTC	55.0	50.0	58.0	57.5	2640-2660 bp	1.0	77.9
ndhF_p3	GATGGAATCGCCCATTACGA	CGAACAGGTTGGTCATCTCA	50.0	50.0	57.8	57.5	2805-2825 bp	1.0	76.7
ndhF_p4	GATGGAATCGCCCATTACGA	ACGAACAGGTTGGTCATCTC	50.0	50.0	57.8	57.5	2806-2826 bp	1.0	76.7
ndhF_p5	ACATGCCGAAGTGATGGAAA	CGAACAGGTTGGTCATCTCA	45.0	50.0	57.8	57.5	2722-2742 bp	1.0	76.5