Hotspot

ndhG-ndhI

SSC · 121360-121744 · 385 bp

Back to Brassaiopsis

Pi 0.0125

MarkerSeek score 65.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 385
variable_sites: 7
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGGCG	50.0	45.0	58.0	57.9	515-535 bp	1.0	83.1
ndhG-ndhI_p2	AATACCACTCCCAGACCTCC	AATCAAATTGCTTTGGGGCG	55.0	45.0	58.1	57.9	541-561 bp	1.0	82.3
ndhG-ndhI_p3	CCACTCCCAGACCTCCTAAT	AATCAAATTGCTTTGGGGCG	55.0	45.0	57.8	57.9	537-557 bp	1.0	81.9
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	GGCGGTTACCAATGTCAGTA	50.0	50.0	58.0	57.6	499-519 bp	1.0	80.9
ndhG-ndhI_p5	TACCACTCCCAGACCTCCTA	AATCAAATTGCTTTGGGGCG	55.0	45.0	58.3	57.9	539-559 bp	1.0	80.8