Hotspot

rpl14-rpl16

LSC · 83428-83566 · 139 bp

Back to Brassaiopsis

Pi 0.024

MarkerSeek score 64.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 5
pi: 0.024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	TGAAATGGGTGGAGTAACAGA	50.0	42.9	58.0	56.6	298-305 bp	1.0	62.6
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	TGAAATGGGTGGAGTAACAGA	50.0	42.9	58.0	56.6	328-335 bp	1.0	62.4
rpl14-rpl16_p3	CCGCTGTTATCCGCTACATT	TGAAATGGGTGGAGTAACAGA	50.0	42.9	58.1	56.6	279-286 bp	1.0	62.0
rpl14-rpl16_p4	ACACATCAATTCTCGAGCCC	TGAAATGGGTGGAGTAACAGAA	50.0	40.9	58.0	57.3	298-305 bp	1.0	60.4
rpl14-rpl16_p5	TCGACGATTGCTAGCTCCTA	TGAAATGGGTGGAGTAACAGAA	50.0	40.9	58.0	57.3	328-335 bp	1.0	60.2