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psbE-petL

LSC · 67027-68191 · 1165 bp

Back to Brassaiopsis

Pi 0.0114

MarkerSeek score 66.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 20
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1302-1310 bp	1.0	81.4
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1304-1312 bp	1.0	81.3
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1225-1233 bp	1.0	80.6
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1227-1235 bp	1.0	80.6
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1311-1319 bp	1.0	80.4