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atpH-atpI

LSC · 14768-15890 · 1123 bp

Back to Brassaiopsis

Pi 0.0101

MarkerSeek score 64.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 17
pi: 0.0101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1205-1212 bp	1.0	77.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1261-1268 bp	1.0	76.7
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	57.9	58.5	1221-1228 bp	1.0	76.4
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1206-1213 bp	1.0	76.2
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	1265-1272 bp	1.0	76.0