Hotspot

atpF-atpH

LSC · 14149-14521 · 373 bp

Back to Brassaiopsis

Pi 0.0161

MarkerSeek score 65.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 9
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	484-490 bp	1.0	79.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	486-492 bp	1.0	78.2
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	550-556 bp	1.0	75.7
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	551-557 bp	1.0	75.6
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	552-558 bp	1.0	75.6