Hotspot

psbK-psbI

LSC · 8698-9116 · 419 bp

Back to Brassaiopsis

Pi 0.0263

MarkerSeek score 70.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 15
pi: 0.0263
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	532-539 bp	1.0	83.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	533-540 bp	1.0	83.2
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	50.0	50.0	57.9	58.3	534-541 bp	1.0	81.4
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.3	58.1	529-536 bp	1.0	81.2
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.3	58.1	530-537 bp	1.0	81.2