Hotspot

ndhF

SSC · 110594-112858 · 2265 bp

Back to Brandisia

Pi 0.00752

MarkerSeek score 44.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2265
variable_sites: 65
pi: 0.00752
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ATTCGAAGCGTTGGAATCGA	TGTCCAATACCCCCTCCTTT	45.0	50.0	58.0	57.9	2833-2892 bp	0.454545	60.4
ndhF_p2	TCGATTCGAAGCGTTGGAAT	TGTCCAATACCCCCTCCTTT	45.0	50.0	58.0	57.9	2836-2895 bp	0.454545	60.4
ndhF_p3	GCGTTGGAATCGACCATTTC	TGTCCAATACCCCCTCCTTT	50.0	50.0	57.8	57.9	2826-2885 bp	0.454545	59.1
ndhF_p4	AAGCGTTGGAATCGACCATT	TGTCCAATACCCCCTCCTTT	45.0	50.0	58.2	57.9	2828-2887 bp	0.454545	59.1
ndhF_p5	AGCGTTGGAATCGACCATTT	TGTCCAATACCCCCTCCTTT	45.0	50.0	58.2	57.9	2827-2886 bp	0.454545	59.1