Hotspot

ndhA

SSC · 119768-121934 · 2167 bp

Back to Brandisia

Pi 0.00882

MarkerSeek score 48.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2167
variable_sites: 68
pi: 0.00882
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGAGATAAGTTGTGA	GCCGGGTTTTATCAACTTGC	33.3	50.0	56.0	58.0	2251-2337 bp	1.0	44.9
ndhA_p2	TCTCTTGTTTGAGATAAGTTGTGA	CGCCGGGTTTTATCAACTTG	33.3	50.0	56.0	57.7	2252-2338 bp	1.0	44.9
ndhA_p3	TCTCTTGTTTGAGATAAGTTGTGAA	GCCGGGTTTTATCAACTTGC	32.0	50.0	56.7	58.0	2251-2337 bp	1.0	44.9
ndhA_p4	CTCTTGTTTGAGATAAGTTGTGA	GCCGGGTTTTATCAACTTGC	34.8	50.0	54.6	58.0	2250-2336 bp	1.0	44.9
ndhA_p5	TCTCTTGTTTGAGATAAGTTGTG	GCCGGGTTTTATCAACTTGC	34.8	50.0	54.6	58.0	2251-2337 bp	1.0	44.9