Hotspot

rpl32-trnL-UAG

SSC · 113485-114296 · 812 bp

Back to Brandisia

Pi 0.0149

MarkerSeek score 65.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 812
variable_sites: 47
pi: 0.0149
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGGAGGGGGTATTGGACA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	859-961 bp	1.0	86.5
rpl32-trnL-UAG_p2	AAAGGAGGGGGTATTGGACA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	852-954 bp	1.0	86.0
rpl32-trnL-UAG_p3	AAAGGAGGGGGTATTGGACA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	858-960 bp	1.0	85.9
rpl32-trnL-UAG_p4	AAAGGAGGGGGTATTGGACA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	857-959 bp	1.0	85.9
rpl32-trnL-UAG_p5	AAAGGAGGGGGTATTGGACA	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.9	58.3	853-955 bp	1.0	85.3