Hotspot

petA-psbJ

LSC · 63988-65035 · 1048 bp

Back to Brandisia

Pi 0.0106

MarkerSeek score 62.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 37
pi: 0.0106
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.6	57.5	1182-1206 bp	1.0	77.0
petA-psbJ_p2	TCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1188-1212 bp	1.0	74.2
petA-psbJ_p3	CCATTACGTGTCCAAGGTCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.2	57.5	1187-1211 bp	1.0	74.2
petA-psbJ_p4	TCGAGAAGGTTCAATTGGCT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.1	57.5	1105-1129 bp	1.0	73.7
petA-psbJ_p5	CGAGAAGGTTCAATTGGCTG	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.1	57.5	1104-1128 bp	1.0	73.3