Hotspot

trnM-CAU-atpE

LSC · 52961-53170 · 210 bp

Back to Brandisia

Pi 0.0124

MarkerSeek score 58.40

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 210
variable_sites: 11
pi: 0.0124
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	316-322 bp	1.0	80.8
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	AGGCTGTCAATGCGATTTCA	50.0	45.0	58.4	58.2	276-282 bp	1.0	80.7
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	AGAGGCTGTCAATGCGATTT	50.0	45.0	58.4	57.9	280-284 bp	0.909091	76.1
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	GTAGAGGCTGTCAATGCGAT	50.0	50.0	58.4	57.8	282-286 bp	0.909091	75.1
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	AGAGGCTGTCAATGCGATTT	50.0	45.0	57.3	57.9	281-285 bp	0.909091	74.0