Hotspot

ndhC-trnV-UAC

LSC · 50894-52051 · 1158 bp

Back to Brandisia

Pi 0.00901
MarkerSeek score 58.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1158
variable_sites
42
pi
0.00901
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1271-1289 bp 1.0 81.7
ndhC-trnV-UAC_p2 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1292-1310 bp 1.0 79.0
ndhC-trnV-UAC_p3 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1270-1288 bp 1.0 79.0
ndhC-trnV-UAC_p4 GGGGCTAAAATCCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1259-1277 bp 1.0 77.9
ndhC-trnV-UAC_p5 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1284-1302 bp 1.0 77.8