Hotspot

trnT-GGU-psbD

LSC · 31719-33028 · 1310 bp

Back to Brandisia

Pi 0.00663
MarkerSeek score 60.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1310
variable_sites
36
pi
0.00663
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA CCGCAACCAGTCATCCATAA 50.0 50.0 57.9 58.0 1448-1460 bp 1.0 82.2
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 50.0 50.0 57.9 58.1 1478-1490 bp 1.0 81.6
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CCGCAACCAGTCATCCATAA 50.0 50.0 58.3 58.0 1411-1423 bp 1.0 80.9
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1468-1480 bp 1.0 80.5
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1441-1453 bp 1.0 80.2