Hotspot

atpF-atpH

LSC · 13411-13790 · 380 bp

Back to Brandisia

Pi 0.00823

MarkerSeek score 57.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 12
pi: 0.00823
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TGGCCCAAAGAAACGAAAGA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	57.9	57.5	532-546 bp	1.0	80.1
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	533-547 bp	1.0	79.5
atpF-atpH_p3	AGTGGCCCAAAGAAACGAAA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	58.2	57.5	534-548 bp	1.0	78.7
atpF-atpH_p4	TTAAATCCGAAACTCCCGGC	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	58.3	57.5	562-576 bp	1.0	78.3
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGT	45.0	47.6	57.9	58.0	526-540 bp	1.0	76.1