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trnH-GUG-psbA

LSC · 89-656 · 568 bp

Back to Brandisia

Pi 0.0341

MarkerSeek score 74.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 57
pi: 0.0341
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTGGCTGCTAT	55.0	55.0	58.1	58.0	562-648 bp	1.0	90.3
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	576-662 bp	1.0	90.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	575-661 bp	1.0	90.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	577-663 bp	1.0	90.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TAGACCTGGCTGCTATCGAA	55.0	50.0	58.1	57.9	558-644 bp	0.909091	84.5