primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG CCTCTAGACCTGGCTGCTAT 20 20 55.0 55.0 58.1 58.0 0.0 0.0 0.137162 89 656 562-648 1.000000 90.3 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA CCTCTAGACCTGGCTGCTAT 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.141524 89 656 576-662 1.000000 90.1 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC CCTCTAGACCTGGCTGCTAT 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.143031 89 656 575-661 1.000000 90.1 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACAATCCACTGCCTTGATCC CCTCTAGACCTGGCTGCTAT 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.143031 89 656 577-663 1.000000 90.1 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 GATCCACTTGGCTACATCCG TAGACCTGGCTGCTATCGAA 20 20 55.0 50.0 58.1 57.9 0.0 0.0 0.196285 89 656 558-644 0.909091 84.5 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 20 21 50.0 38.1 58.0 55.5 0.0 0.0 3.540425 8760 9441 751-766 1.000000 54.4 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 20 21 50.0 38.1 58.0 55.5 0.0 0.0 3.541813 8760 9441 750-765 1.000000 54.4 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 20 23 50.0 39.1 58.0 57.3 0.0 0.0 3.673141 8760 9441 754-769 1.000000 53.4 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 20 23 50.0 39.1 58.0 57.3 0.0 0.0 3.673141 8760 9441 753-768 1.000000 53.4 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 20 23 50.0 39.1 58.0 57.3 0.0 0.0 3.674529 8760 9441 752-767 1.000000 53.4 atpA-atpF_p1 atpA-atpF 1 AATTTCGTCGGCTCGAATGG TTAGCATGTTGGGAGCGATG 20 20 50.0 50.0 59.0 58.3 13.2 0.0 1.325932 12078 12159 138-179 1.000000 80.4 atpA-atpF_p2 atpA-atpF 2 AATTTCGTCGGCTCGAATGG TGTTGGGAGCGATGAAAGAA 20 20 50.0 45.0 59.0 57.4 13.2 0.0 1.552865 12078 12159 132-173 1.000000 78.9 atpA-atpF_p3 atpA-atpF 3 GTCGGCTCGAATGGTTACC TTAGCATGTTGGGAGCGATG 19 20 57.9 50.0 58.3 58.3 0.0 0.0 1.658468 12078 12159 132-173 1.000000 78.0 atpA-atpF_p4 atpA-atpF 4 AATTTCGTCGGCTCGAATGG ATTAGCATGTTGGGAGCGAT 20 20 50.0 45.0 59.0 57.4 13.2 0.0 1.639006 12078 12159 139-180 1.000000 77.8 atpA-atpF_p5 atpA-atpF 5 ATTTCGTCGGCTCGAATGG TTAGCATGTTGGGAGCGATG 19 20 52.6 50.0 58.3 58.3 13.2 0.0 1.654793 12078 12159 137-178 1.000000 77.8 atpF-atpH_p1 atpF-atpH 1 TGGCCCAAAGAAACGAAAGA CCTGAGGCAGAGGGAAAAAT 20 20 45.0 50.0 57.9 57.5 0.0 0.0 0.581597 13411 13790 532-546 1.000000 80.1 atpF-atpH_p2 atpF-atpH 2 GTGGCCCAAAGAAACGAAAG CCTGAGGCAGAGGGAAAAAT 20 20 50.0 50.0 57.9 57.5 0.0 0.0 0.655748 13411 13790 533-547 1.000000 79.5 atpF-atpH_p3 atpF-atpH 3 AGTGGCCCAAAGAAACGAAA CCTGAGGCAGAGGGAAAAAT 20 20 45.0 50.0 58.2 57.5 0.0 0.0 0.751252 13411 13790 534-548 1.000000 78.7 atpF-atpH_p4 atpF-atpH 4 TTAAATCCGAAACTCCCGGC CCTGAGGCAGAGGGAAAAAT 20 20 50.0 50.0 58.3 57.5 0.0 0.0 0.780019 13411 13790 562-576 1.000000 78.3 atpF-atpH_p5 atpF-atpH 5 TGGCCCAAAGAAACGAAAGA GCAGAGGGAAAAATACGAGGT 20 21 45.0 47.6 57.9 58.0 0.0 0.0 1.072818 13411 13790 526-540 1.000000 76.1 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GCCCTTTTAACTCAGCGGTA CCGCAACCAGTCATCCATAA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.140495 31719 33028 1448-1460 1.000000 82.2 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.216651 31719 33028 1478-1490 1.000000 81.6 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 GGCGTAAGTCATCGGTTCAA CCGCAACCAGTCATCCATAA 20 20 50.0 50.0 58.3 58.0 0.0 0.0 0.319764 31719 33028 1411-1423 1.000000 80.9 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 57.9 58.2 0.0 0.0 0.349607 31719 33028 1468-1480 1.000000 80.5 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 20 20 50.0 50.0 58.3 58.1 0.0 0.0 0.395920 31719 33028 1441-1453 1.000000 80.2 psaB-psaA_p1 psaB-psaA 1 AACCCTTGGCTAAACCTTGG GTACAAGGACGTGCTGTAGG 20 20 50.0 55.0 58.0 58.0 0.0 11.4 0.017512 39637 39661 156-156 1.000000 81.4 psaB-psaA_p2 psaB-psaA 2 AACCCTTGGCTAAACCTTGG ACAACATGGGCGTTCTTCTT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.028425 39637 39661 105-105 1.000000 81.3 psaB-psaA_p3 psaB-psaA 3 AACCCTTGGCTAAACCTTGG TACAAGGACGTGCTGTAGGA 20 20 50.0 50.0 58.0 58.1 0.0 11.4 0.095904 39637 39661 155-155 1.000000 80.8 psaB-psaA_p4 psaB-psaA 4 AACCCTTGGCTAAACCTTGG GGGCGTTCTTCTTAGCAAGA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.104444 39637 39661 98-98 1.000000 80.8 psaB-psaA_p5 psaB-psaA 5 AATACGACGAGTAGTGGGGT GTACAAGGACGTGCTGTAGG 20 20 50.0 55.0 57.9 58.0 0.0 11.4 0.142619 39637 39661 184-184 1.000000 80.2 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 GGGGCTAAAATCCCGGAAAT AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 57.9 57.8 0.7 0.0 0.279423 50894 52051 1271-1289 1.000000 81.7 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 20 20 50.0 50.0 58.4 57.8 0.0 0.0 0.605515 50894 52051 1292-1310 1.000000 79.0 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 GGGGCTAAAATCCCGGAAAT GAAGGTCTACGGTTCGAGTC 20 20 50.0 55.0 57.9 57.5 0.7 0.0 0.623195 50894 52051 1270-1288 1.000000 79.0 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 GGGGCTAAAATCCCGGAAAT GTTCGAGTCCGTATAGCCCT 20 20 50.0 55.0 57.9 58.7 0.7 0.0 0.765489 50894 52051 1259-1277 1.000000 77.9 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 20 20 55.0 50.0 58.6 57.8 0.0 0.0 0.766291 50894 52051 1284-1302 1.000000 77.8 trnM-CAU-atpE_p1 trnM-CAU-atpE 1 TATTGCTTTCATACGGCGGG GAGGCAAATCTAGCTCTCCG 20 20 50.0 55.0 58.4 57.9 13.2 0.0 0.558762 52961 53170 316-322 1.000000 80.8 trnM-CAU-atpE_p2 trnM-CAU-atpE 2 TATTGCTTTCATACGGCGGG AGGCTGTCAATGCGATTTCA 20 20 50.0 45.0 58.4 58.2 13.2 0.0 0.586236 52961 53170 276-282 1.000000 80.7 trnM-CAU-atpE_p3 trnM-CAU-atpE 3 TATTGCTTTCATACGGCGGG AGAGGCTGTCAATGCGATTT 20 20 50.0 45.0 58.4 57.9 13.2 0.0 0.531127 52961 53170 280-284 0.909091 76.1 trnM-CAU-atpE_p4 trnM-CAU-atpE 4 TATTGCTTTCATACGGCGGG GTAGAGGCTGTCAATGCGAT 20 20 50.0 50.0 58.4 57.8 13.2 0.0 0.642541 52961 53170 282-286 0.909091 75.1 trnM-CAU-atpE_p5 trnM-CAU-atpE 5 GTATTGCTTTCATACGGCGG AGAGGCTGTCAATGCGATTT 20 20 50.0 45.0 57.3 57.9 13.2 0.0 0.783080 52961 53170 281-285 0.909091 74.0 petA-psbJ_p1 petA-psbJ 1 ACGTGTCCAAGGTCTTTTGT TCCGGATTAGGTTCATCCCT 20 20 45.0 50.0 57.6 57.5 0.0 0.0 0.902837 63988 65035 1182-1206 1.000000 77.0 petA-psbJ_p2 petA-psbJ 2 TCCATTACGTGTCCAAGGTC TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 57.2 57.5 0.0 0.0 1.244474 63988 65035 1188-1212 1.000000 74.2 petA-psbJ_p3 petA-psbJ 3 CCATTACGTGTCCAAGGTCT TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 57.2 57.5 0.0 0.0 1.252063 63988 65035 1187-1211 1.000000 74.2 petA-psbJ_p4 petA-psbJ 4 TCGAGAAGGTTCAATTGGCT TCCGGATTAGGTTCATCCCT 20 20 45.0 50.0 57.1 57.5 5.4 0.0 1.348693 63988 65035 1105-1129 1.000000 73.7 petA-psbJ_p5 petA-psbJ 5 CGAGAAGGTTCAATTGGCTG TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 57.1 57.5 5.4 0.0 1.397438 63988 65035 1104-1128 1.000000 73.3 psbE-petL_p1 psbE-petL 1 TGACGAATAACCAACCTGCA GTCGTATCTTGCTCAGACCA 20 20 45.0 50.0 57.4 57.1 0.0 0.0 1.494052 65818 66721 1107-1149 1.000000 71.7 psbE-petL_p2 psbE-petL 2 ACGTTCTCCTGTGTTTCCAG GTCGTATCTTGCTCAGACCA 20 20 50.0 50.0 57.8 57.1 0.0 0.0 1.181946 65818 66721 1016-1058 0.909091 70.3 psbE-petL_p3 psbE-petL 3 CGTTCTCCTGTGTTTCCAGA GTCGTATCTTGCTCAGACCA 20 20 50.0 50.0 57.5 57.1 0.0 0.0 1.467757 65818 66721 1015-1057 0.909091 68.1 psbE-petL_p4 psbE-petL 4 ACGTTCTCCTGTGTTTCCAG GTCGTATCTTGCTCAGACCAA 20 21 50.0 47.6 57.8 57.8 0.0 0.0 1.472706 65818 66721 1016-1058 0.909091 68.0 psbE-petL_p5 psbE-petL 5 ACGTTCTCCTGTGTTTCCAG AGTCGTATCTTGCTCAGACC 20 20 50.0 50.0 57.8 56.8 0.0 0.0 1.478004 65818 66721 1017-1059 0.909091 68.0 psbB-psbT_p1 psbB-psbT 1 TTTTTGCCGGTATTGACCCA TGGAACTTTAGGTGGTTCTCG 20 21 45.0 47.6 58.0 57.6 1.9 0.0 1.399462 74713 74905 370-391 1.000000 73.0 psbB-psbT_p2 psbB-psbT 2 GCCGGTATTGACCCAGATTT TGGAACTTTAGGTGGTTCTCG 20 21 50.0 47.6 57.9 57.6 1.9 0.0 1.449043 74713 74905 365-386 1.000000 72.6 psbB-psbT_p3 psbB-psbT 3 TTTTTGCCGGTATTGACCCA GGAACTTTAGGTGGTTCTCGA 20 21 45.0 47.6 58.0 57.3 1.9 0.0 1.675968 74713 74905 369-390 1.000000 70.8 psbB-psbT_p4 psbB-psbT 4 TCAGAGATGTTTTTGCCGGT TGGAACTTTAGGTGGTTCTCG 20 21 45.0 47.6 57.7 57.6 1.9 0.0 1.666612 74713 74905 379-400 1.000000 70.7 psbB-psbT_p5 psbB-psbT 5 GCCGGTATTGACCCAGATTT GGAACTTTAGGTGGTTCTCGA 20 21 50.0 47.6 57.9 57.3 1.9 0.0 1.725549 74713 74905 364-385 1.000000 70.4 psbT-psbN_p1 psbT-psbN 1 CGAGAACCACCTAAAGTTCCA GGGTACGCCTTATATACCGC 21 20 47.6 55.0 57.6 57.7 0.0 0.0 1.680523 75014 75073 174-174 1.000000 69.8 psbT-psbN_p2 psbT-psbN 2 CGAGAACCACCTAAAGTTCCA GATCCATTCGAGGAACACGG 21 20 47.6 55.0 57.6 58.4 0.0 0.0 1.824342 75014 75073 126-126 1.000000 69.8 psbT-psbN_p3 psbT-psbN 3 CGAGAACCACCTAAAGTTCCA GTTTTACCGGGTACGCCTTA 21 20 47.6 50.0 57.6 57.6 0.0 6.3 1.774705 75014 75073 182-182 1.000000 68.9 psbT-psbN_p4 psbT-psbN 4 CGAGAACCACCTAAAGTTCCA TAAGTTTTACCGGGTACGCC 21 20 47.6 50.0 57.6 57.6 0.0 6.3 1.774705 75014 75073 185-185 1.000000 68.8 psbT-psbN_p5 psbT-psbN 5 CGAGAACCACCTAAAGTTCCA CGGGTACGCCTTATATACCG 21 20 47.6 55.0 57.6 57.5 0.0 4.1 1.945894 75014 75073 175-175 1.000000 67.6 rpl22_p1 rpl22 1 GTTGTGCAAACCAAAGGGAA TCCGAGGACATGCAAAAAGT 20 20 45.0 45.0 57.3 57.7 0.0 0.0 0.952507 83953 84423 625-631 1.000000 76.3 rpl22_p2 rpl22 2 GTTGTGCAAACCAAAGGGAA CCGAGGACATGCAAAAAGTG 20 20 45.0 50.0 57.3 57.7 0.0 0.0 1.018710 83953 84423 624-630 1.000000 75.8 rpl22_p3 rpl22 3 TGTGCAAACCAAAGGGAATG TCCGAGGACATGCAAAAAGT 20 20 45.0 45.0 57.1 57.7 0.0 0.0 1.177043 83953 84423 623-629 1.000000 74.5 rpl22_p4 rpl22 4 TGTGCAAACCAAAGGGAATG CCGAGGACATGCAAAAAGTG 20 20 45.0 50.0 57.1 57.7 0.0 0.0 1.243246 83953 84423 622-628 1.000000 74.0 rpl22_p5 rpl22 5 CCAAGTCTGAAACCAAGTGGA TCCGAGGACATGCAAAAAGT 21 20 47.6 45.0 58.1 57.7 3.8 0.0 1.339402 83953 84423 587-593 1.000000 73.3 ndhF_p1 ndhF 1 ATTCGAAGCGTTGGAATCGA TGTCCAATACCCCCTCCTTT 20 20 45.0 50.0 58.0 57.9 3.3 0.0 0.073414 110594 112858 2833-2892 0.454545 60.4 ndhF_p2 ndhF 2 TCGATTCGAAGCGTTGGAAT TGTCCAATACCCCCTCCTTT 20 20 45.0 50.0 58.0 57.9 31.8 0.0 0.073414 110594 112858 2836-2895 0.454545 60.4 ndhF_p3 ndhF 3 GCGTTGGAATCGACCATTTC TGTCCAATACCCCCTCCTTT 20 20 50.0 50.0 57.8 57.9 0.0 0.0 0.242892 110594 112858 2826-2885 0.454545 59.1 ndhF_p4 ndhF 4 AAGCGTTGGAATCGACCATT TGTCCAATACCCCCTCCTTT 20 20 45.0 50.0 58.2 57.9 0.0 0.0 0.247615 110594 112858 2828-2887 0.454545 59.1 ndhF_p5 ndhF 5 AGCGTTGGAATCGACCATTT TGTCCAATACCCCCTCCTTT 20 20 45.0 50.0 58.2 57.9 0.0 0.0 0.247615 110594 112858 2827-2886 0.454545 59.1 ndhF-rpl32_p1 ndhF-rpl32 1 CTGGAAGTGGAACGAAAGGT TGATGCAGAAGTACGTTTTT 20 20 50.0 35.0 57.7 53.5 0.0 0.0 4.792232 112859 113322 519-561 1.000000 48.6 ndhF-rpl32_p2 ndhF-rpl32 2 CTGGAAGTGGAACGAAAGGT TTGATGCAGAAGTACGTTTT 20 20 50.0 35.0 57.7 53.5 0.0 0.0 4.792232 112859 113322 520-562 1.000000 48.6 ndhF-rpl32_p3 ndhF-rpl32 3 CTGGAAGTGGAACGAAAGGT TTTGATGCAGAAGTACGTTT 20 20 50.0 35.0 57.7 53.5 0.0 0.0 4.792232 112859 113322 521-563 1.000000 48.6 ndhF-rpl32_p4 ndhF-rpl32 4 CTGGAAGTGGAACGAAAGGT TTTTGATGCAGAAGTACGTT 20 20 50.0 35.0 57.7 53.5 0.0 0.0 4.792232 112859 113322 522-564 1.000000 48.6 ndhF-rpl32_p5 ndhF-rpl32 5 CTGGAAGTGGAACGAAAGGT TTTTTGATGCAGAAGTACGT 20 20 50.0 35.0 57.7 53.5 0.0 0.0 4.792232 112859 113322 523-565 1.000000 48.6 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AAAGGAGGGGGTATTGGACA TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.193109 113485 114296 859-961 1.000000 86.5 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AAAGGAGGGGGTATTGGACA AGCAGCGTGTCTACCAATTT 20 20 50.0 45.0 57.9 57.8 0.0 0.0 0.253836 113485 114296 852-954 1.000000 86.0 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAAGGAGGGGGTATTGGACA CCTAAGAGCAGCGTGTCTAC 20 20 50.0 55.0 57.9 57.8 0.0 0.0 0.265126 113485 114296 858-960 1.000000 85.9 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AAAGGAGGGGGTATTGGACA CTAAGAGCAGCGTGTCTACC 20 20 50.0 55.0 57.9 57.8 0.0 0.0 0.265126 113485 114296 857-959 1.000000 85.9 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AAAGGAGGGGGTATTGGACA GAGCAGCGTGTCTACCAATT 20 20 50.0 50.0 57.9 58.3 0.0 0.0 0.341978 113485 114296 853-955 1.000000 85.3 ndhA_p1 ndhA 1 TCTCTTGTTTGAGATAAGTTGTGA GCCGGGTTTTATCAACTTGC 24 20 33.3 50.0 56.0 58.0 0.0 2.2 5.996498 119768 121934 2251-2337 1.000000 44.9 ndhA_p2 ndhA 2 TCTCTTGTTTGAGATAAGTTGTGA CGCCGGGTTTTATCAACTTG 24 20 33.3 50.0 56.0 57.7 0.0 2.2 6.243742 119768 121934 2252-2338 1.000000 44.9 ndhA_p3 ndhA 3 TCTCTTGTTTGAGATAAGTTGTGAA GCCGGGTTTTATCAACTTGC 25 20 32.0 50.0 56.7 58.0 0.0 2.2 6.343375 119768 121934 2251-2337 1.000000 44.9 ndhA_p4 ndhA 4 CTCTTGTTTGAGATAAGTTGTGA GCCGGGTTTTATCAACTTGC 23 20 34.8 50.0 54.6 58.0 0.0 2.2 6.393678 119768 121934 2250-2336 1.000000 44.9 ndhA_p5 ndhA 5 TCTCTTGTTTGAGATAAGTTGTG GCCGGGTTTTATCAACTTGC 23 20 34.8 50.0 54.6 58.0 0.0 2.2 6.393678 119768 121934 2251-2337 1.000000 44.9 ycf1_p1 ycf1 1 TTTCGAGTGTAGGTGGGGAT AAGAGGGATCCACCGAAGAA 20 20 50.0 50.0 58.1 58.0 0.0 15.2 0.100394 128278 128397 492-522 1.000000 83.0 ycf1_p2 ycf1 2 TTTCGAGTGTAGGTGGGGAT ACAGGAACAAGAGGGATCCA 20 20 50.0 50.0 58.1 58.0 0.0 15.2 0.097808 128278 128397 500-530 1.000000 82.9 ycf1_p3 ycf1 3 TAAAGCCAAGGGTTGCAGAG ACAGGAACAAGAGGGATCCA 20 20 50.0 50.0 58.1 58.0 4.0 15.2 0.135093 128278 128397 537-567 1.000000 82.7 ycf1_p4 ycf1 4 TTTCGAGTGTAGGTGGGGAT GCAGAATACCGTCACCCATT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.091402 128278 128397 624-654 1.000000 82.6 ycf1_p5 ycf1 5 TAAAGCCAAGGGTTGCAGAG GCAGAATACCGTCACCCATT 20 20 50.0 50.0 58.1 58.0 4.0 0.0 0.128687 128278 128397 661-691 1.000000 82.4