Hotspot

ndhI

SSC · 122260-122802 · 543 bp

Pi 0.218

MarkerSeek score 61.90

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 543
variable_sites: 207
pi: 0.218
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TTTTGGGGGTGGAATCTGTG	ACAAACCAGTGAAGTGACCC	50.0	50.0	58.0	57.9	1760 bp	0.5	59.6
ndhI_p2	TTTTGGGGGTGGAATCTGTG	TCCCAACGAAAAGGCAGAAA	50.0	45.0	58.0	57.9	1568 bp	0.5	59.5
ndhI_p3	GGTTTTTGGGGGTGGAATCT	ACAAACCAGTGAAGTGACCC	50.0	50.0	58.0	57.9	1763 bp	0.5	59.4
ndhI_p4	TTTTGGGGGTGGAATCTGTG	GGTCCAGGAAAGGAAAAGGG	50.0	55.0	58.0	58.1	1394 bp	0.5	59.4
ndhI_p5	TTTTGGGGGTGGAATCTGTG	GTCCAGGAAAGGAAAAGGGG	50.0	55.0	58.0	58.1	1393 bp	0.5	59.4