Hotspot

ndhG

SSC · 123134-123664 · 531 bp

Back to Brahea

Pi 0.224
MarkerSeek score 59.80
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
208
pi
0.224
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 CCCCTTTTCCTTTCCTGGAC AGCCGCAACAGCTATAACAA 55.0 45.0 58.1 57.9 1040-1056 bp 0.5 59.8
ndhG_p2 CCCTTTTCCTTTCCTGGACC AGCCGCAACAGCTATAACAA 55.0 45.0 58.1 57.9 1039-1055 bp 0.5 59.8
ndhG_p3 TATGTGTTCGTGTATGCCCG AGCCGCAACAGCTATAACAA 50.0 45.0 58.1 57.9 1634-1650 bp 0.5 59.4
ndhG_p4 GTGTTCGTGTATGCCCGATA AGCCGCAACAGCTATAACAA 50.0 45.0 57.8 57.9 1631-1647 bp 0.5 58.3
ndhG_p5 TCAAATTGCTTTGGGTCGGT AGCCGCAACAGCTATAACAA 45.0 45.0 58.3 57.9 1426-1442 bp 0.5 57.7