Hotspot

trnL-UAG-rpl32

SSC · 127877-128661 · 785 bp

Back to Brahea

Pi 0.221
MarkerSeek score 76.60
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
785
variable_sites
306
pi
0.221
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.87
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AGATTGGCCGAAAATCCCAT 50.0 45.0 58.0 57.5 1514-1533 bp 0.5 57.1
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AGATTGGCCGAAAATCCCAT 50.0 45.0 58.0 57.5 1515-1534 bp 0.5 57.1
trnL-UAG-rpl32_p3 ATCCGCATAAAAGAGCTGCA AGATTGGCCGAAAATCCCAT 45.0 45.0 58.0 57.5 2102-2121 bp 0.5 56.6
trnL-UAG-rpl32_p4 TCCGCATAAAAGAGCTGCAT AGATTGGCCGAAAATCCCAT 45.0 45.0 58.0 57.5 2101-2120 bp 0.5 56.6
trnL-UAG-rpl32_p5 AGCGTGTCTACCGATTTCAC ACAGATTGGCCGAAAATCCC 50.0 50.0 58.0 58.5 1516-1535 bp 0.5 56.6