Hotspot

ndhD-ccsA

SSC · 126538-126753 · 216 bp

Back to Brahea

Pi 0.245
MarkerSeek score 63.30
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
216
variable_sites
94
pi
0.245
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TTCTCGATGGCTTCTCTTGC CTTATTGGAGTTGGGACCCG 50.0 55.0 58.0 58.0 797 bp 0.5 60.0
ndhD-ccsA_p2 TTCTCGATGGCTTCTCTTGC ATGCAGCTCTTTTATGCGGA 50.0 45.0 58.0 58.0 1106 bp 0.5 59.8
ndhD-ccsA_p3 TTCTCGATGGCTTCTCTTGC TGCAGCTCTTTTATGCGGAT 50.0 45.0 58.0 58.0 1105 bp 0.5 59.8
ndhD-ccsA_p4 CTCGATGGCTTCTCTTGCAT CTTATTGGAGTTGGGACCCG 50.0 55.0 58.0 58.0 795 bp 0.5 59.7
ndhD-ccsA_p5 CTCGATGGCTTCTCTTGCAT TGCAGCTCTTTTATGCGGAT 50.0 45.0 58.0 58.0 1103 bp 0.5 59.6