Hotspot

ndhI-ndhG

SSC · 122803-123133 · 331 bp

Back to Brahea

Pi 0.182
MarkerSeek score 59.90
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
331
variable_sites
105
pi
0.182
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.80
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 TATGTGTTCGTGTATGCCCG ACAAACCAGTGAAGTGACCC 50.0 50.0 58.1 57.9 981 bp 0.5 59.1
ndhI-ndhG_p2 TATGTGTTCGTGTATGCCCG TCCCAACGAAAAGGCAGAAA 50.0 45.0 58.1 57.9 789 bp 0.5 59.1
ndhI-ndhG_p3 TATGTGTTCGTGTATGCCCG TGTGCGGCAGCTACAAAATA 50.0 45.0 58.1 58.2 854 bp 0.5 58.0
ndhI-ndhG_p4 GTGTTCGTGTATGCCCGATA ACAAACCAGTGAAGTGACCC 50.0 50.0 57.8 57.9 978 bp 0.5 58.0
ndhI-ndhG_p5 GTGTTCGTGTATGCCCGATA TCCCAACGAAAAGGCAGAAA 50.0 45.0 57.8 57.9 786 bp 0.5 57.9