Hotspot

ycf1-rps15

SSC · 118078-118465 · 388 bp

Back to Brahea

Pi 0.252
MarkerSeek score 68.70
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
SSC
length_bp
388
variable_sites
172
pi
0.252
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 ACGGCAATATGCTTGTGGAT GGCTCAGCTCCAGCATAATT 45.0 50.0 57.9 58.0 1456-1458 bp 0.5 60.0
ycf1-rps15_p2 ACGGCAATATGCTTGTGGAT AACACCATGCATTGATGGGT 45.0 45.0 57.9 58.1 1203-1205 bp 0.5 59.8
ycf1-rps15_p3 ACGGCAATATGCTTGTGGAT ACCATGCATTGATGGGTGTT 45.0 45.0 57.9 58.1 1200-1202 bp 0.5 59.8
ycf1-rps15_p4 ACGGCAATATGCTTGTGGAT GAAGCTATACGGCTCAGCTC 45.0 55.0 57.9 57.9 1466-1468 bp 0.5 59.7
ycf1-rps15_p5 ACGGCAATATGCTTGTGGAT GAGAAGCTATACGGCTCAGC 45.0 55.0 57.9 57.9 1468-1470 bp 0.5 59.7