primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ycf1	1	TCGATCCATGAATTGGGCAA	CACTGCCACCGCATAGTAAT	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.480103	116060	116179	871-898	0.500000	59.4
ycf1_p2	ycf1	2	TTCGATCCATGAATTGGGCA	CACTGCCACCGCATAGTAAT	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.480103	116060	116179	872-899	0.500000	59.4
ycf1_p3	ycf1	3	TCGATCCATGAATTGGGCAA	TCAATGAATCCACTGCCACC	20	20	45.0	50.0	57.6	58.2	0.0	0.0	0.595550	116060	116179	881-908	0.500000	58.5
ycf1_p4	ycf1	4	TTCGATCCATGAATTGGGCA	TCAATGAATCCACTGCCACC	20	20	45.0	50.0	57.6	58.2	0.0	0.0	0.595550	116060	116179	882-909	0.500000	58.5
ycf1_p5	ycf1	5	TCGATCCATGAATTGGGCAA	TCGTCAATGAATCCACTGCC	20	20	45.0	50.0	57.6	58.3	0.0	0.0	0.695824	116060	116179	884-911	0.500000	57.7
ycf1-rps15_p1	ycf1-rps15	1	ACGGCAATATGCTTGTGGAT	GGCTCAGCTCCAGCATAATT	20	20	45.0	50.0	57.9	58.0	2.0	14.7	0.078683	118078	118465	1456-1458	0.500000	60.0
ycf1-rps15_p2	ycf1-rps15	2	ACGGCAATATGCTTGTGGAT	AACACCATGCATTGATGGGT	20	20	45.0	45.0	57.9	58.1	2.0	8.9	0.115760	118078	118465	1203-1205	0.500000	59.8
ycf1-rps15_p3	ycf1-rps15	3	ACGGCAATATGCTTGTGGAT	ACCATGCATTGATGGGTGTT	20	20	45.0	45.0	57.9	58.1	2.0	8.9	0.115760	118078	118465	1200-1202	0.500000	59.8
ycf1-rps15_p4	ycf1-rps15	4	ACGGCAATATGCTTGTGGAT	GAAGCTATACGGCTCAGCTC	20	20	45.0	55.0	57.9	57.9	2.0	0.0	0.116051	118078	118465	1466-1468	0.500000	59.7
ycf1-rps15_p5	ycf1-rps15	5	ACGGCAATATGCTTGTGGAT	GAGAAGCTATACGGCTCAGC	20	20	45.0	55.0	57.9	57.9	2.0	0.0	0.116051	118078	118465	1468-1470	0.500000	59.7
ndhH_p1	ndhH	1	CAGTTGGATATTCGGGAGCC	CCAGCATATTCAGGCCCAAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.121135	118846	120027	1524-1526	0.500000	59.3
ndhH_p2	ndhH	2	GGCTCCTGGCTTATTTGTCA	CCAGCATATTCAGGCCCAAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.134141	118846	120027	1580-1582	0.500000	59.2
ndhH_p3	ndhH	3	CAGTTGGATATTCGGGAGCC	CCAACGGACCAGCATATTCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.152722	118846	120027	1532-1534	0.500000	59.0
ndhH_p4	ndhH	4	GGCTCCTGGCTTATTTGTCA	CCAACGGACCAGCATATTCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165728	118846	120027	1588-1590	0.500000	58.9
ndhH_p5	ndhH	5	CAGTTGGATATTCGGGAGCC	TGTTCCAACGAATCACACGT	20	20	55.0	45.0	58.1	58.1	0.0	0.0	0.173087	118846	120027	1892-1894	0.500000	58.8
ndhA_p1	ndhA	1	TGTCAGAGTAGAAGCCCCAA	CGGGCATACACGAACACATA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.094945	120029	122171	2659-2660	0.500000	59.2
ndhA_p2	ndhA	2	ACTTTCCCCTGGAGATGGAA	CGGGCATACACGAACACATA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.130526	120029	122171	2601-2602	0.500000	59.0
ndhA_p3	ndhA	3	TGGGAAGTCCAATGGCAAAA	CGGGCATACACGAACACATA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.164870	120029	122171	2901-2902	0.500000	58.7
ndhA_p4	ndhA	4	GGGCATTATTGGTGGAGAGG	ACCTTGCAGCTCGTATTGTT	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.198122	120029	122171	2858-2859	0.500000	58.5
ndhA_p5	ndhA	5	TGTCAGAGTAGAAGCCCCAA	ACCTTGCAGCTCGTATTGTT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.209180	120029	122171	2495-2496	0.500000	58.3
ndhI_p1	ndhI	1	TTTTGGGGGTGGAATCTGTG	ACAAACCAGTGAAGTGACCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.061025	122260	122802	1760-1760	0.500000	59.6
ndhI_p2	ndhI	2	TTTTGGGGGTGGAATCTGTG	TCCCAACGAAAAGGCAGAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.068293	122260	122802	1568-1568	0.500000	59.5
ndhI_p3	ndhI	3	GGTTTTTGGGGGTGGAATCT	ACAAACCAGTGAAGTGACCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.076958	122260	122802	1763-1763	0.500000	59.4
ndhI_p4	ndhI	4	TTTTGGGGGTGGAATCTGTG	GGTCCAGGAAAGGAAAAGGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.083511	122260	122802	1394-1394	0.500000	59.4
ndhI_p5	ndhI	5	TTTTGGGGGTGGAATCTGTG	GTCCAGGAAAGGAAAAGGGG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.083511	122260	122802	1393-1393	0.500000	59.4
ndhI-ndhG_p1	ndhI-ndhG	1	TATGTGTTCGTGTATGCCCG	ACAAACCAGTGAAGTGACCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.123552	122803	123133	981-981	0.500000	59.1
ndhI-ndhG_p2	ndhI-ndhG	2	TATGTGTTCGTGTATGCCCG	TCCCAACGAAAAGGCAGAAA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.130820	122803	123133	789-789	0.500000	59.1
ndhI-ndhG_p3	ndhI-ndhG	3	TATGTGTTCGTGTATGCCCG	TGTGCGGCAGCTACAAAATA	20	20	50.0	45.0	58.1	58.2	0.0	10.9	0.255977	122803	123133	854-854	0.500000	58.0
ndhI-ndhG_p4	ndhI-ndhG	4	GTGTTCGTGTATGCCCGATA	ACAAACCAGTGAAGTGACCC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.265651	122803	123133	978-978	0.500000	58.0
ndhI-ndhG_p5	ndhI-ndhG	5	GTGTTCGTGTATGCCCGATA	TCCCAACGAAAAGGCAGAAA	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.272919	122803	123133	786-786	0.500000	57.9
ndhG_p1	ndhG	1	CCCCTTTTCCTTTCCTGGAC	AGCCGCAACAGCTATAACAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.183219	123134	123664	1040-1056	0.500000	59.8
ndhG_p2	ndhG	2	CCCTTTTCCTTTCCTGGACC	AGCCGCAACAGCTATAACAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.183219	123134	123664	1039-1055	0.500000	59.8
ndhG_p3	ndhG	3	TATGTGTTCGTGTATGCCCG	AGCCGCAACAGCTATAACAA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.178720	123134	123664	1634-1650	0.500000	59.4
ndhG_p4	ndhG	4	GTGTTCGTGTATGCCCGATA	AGCCGCAACAGCTATAACAA	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.320819	123134	123664	1631-1647	0.500000	58.3
ndhG_p5	ndhG	5	TCAAATTGCTTTGGGTCGGT	AGCCGCAACAGCTATAACAA	20	20	45.0	45.0	58.3	57.9	0.0	0.0	0.409444	123134	123664	1426-1442	0.500000	57.7
ndhE-psaC_p1	ndhE-psaC	1	CTGAAGCAGCTATTGGGCTA	ATCGAGTACGGGCTTTTGTC	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.633485	124208	124667	805-1123	0.500000	59.3
ndhE-psaC_p2	ndhE-psaC	2	TGAAGCAGCTATTGGGCTAG	ATCGAGTACGGGCTTTTGTC	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.633485	124208	124667	804-1122	0.500000	59.3
ndhE-psaC_p3	ndhE-psaC	3	TGTTATAGCTGTTGCGGCTG	ATCGAGTACGGGCTTTTGTC	20	20	50.0	50.0	58.6	58.0	12.2	0.0	0.637329	124208	124667	825-1143	0.500000	59.2
ndhE-psaC_p4	ndhE-psaC	4	CTGAAGCAGCTATTGGGCTA	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.753385	124208	124667	596-914	0.500000	58.4
ndhE-psaC_p5	ndhE-psaC	5	TGAAGCAGCTATTGGGCTAG	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	57.4	58.1	0.0	0.0	0.753385	124208	124667	595-913	0.500000	58.4
ndhD_p1	ndhD	1	GACAAAAGCCCGTACTCGAT	CTTATTGGAGTTGGGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	5.9	0.044357	125032	126537	2013-2013	0.500000	59.8
ndhD_p2	ndhD	2	GACAAAAGCCCGTACTCGAT	ATGCAGCTCTTTTATGCGGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048657	125032	126537	2322-2322	0.500000	59.7
ndhD_p3	ndhD	3	GACAAAAGCCCGTACTCGAT	TGCAGCTCTTTTATGCGGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048657	125032	126537	2321-2321	0.500000	59.7
ndhD_p4	ndhD	4	CTGCTTCGTCTGGTGTCTAC	CTTATTGGAGTTGGGACCCG	20	20	55.0	55.0	58.0	58.0	0.0	5.9	0.054414	125032	126537	2410-2410	0.500000	59.7
ndhD_p5	ndhD	5	CTGCTTCGTCTGGTGTCTAC	TGCAGCTCTTTTATGCGGAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.058714	125032	126537	2718-2718	0.500000	59.6
ndhD-ccsA_p1	ndhD-ccsA	1	TTCTCGATGGCTTCTCTTGC	CTTATTGGAGTTGGGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	5.9	0.054123	126538	126753	797-797	0.500000	60.0
ndhD-ccsA_p2	ndhD-ccsA	2	TTCTCGATGGCTTCTCTTGC	ATGCAGCTCTTTTATGCGGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.058422	126538	126753	1106-1106	0.500000	59.8
ndhD-ccsA_p3	ndhD-ccsA	3	TTCTCGATGGCTTCTCTTGC	TGCAGCTCTTTTATGCGGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.058422	126538	126753	1105-1105	0.500000	59.8
ndhD-ccsA_p4	ndhD-ccsA	4	CTCGATGGCTTCTCTTGCAT	CTTATTGGAGTTGGGACCCG	20	20	50.0	55.0	58.0	58.0	0.0	5.9	0.087559	126538	126753	795-795	0.500000	59.7
ndhD-ccsA_p5	ndhD-ccsA	5	CTCGATGGCTTCTCTTGCAT	TGCAGCTCTTTTATGCGGAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.091858	126538	126753	1103-1103	0.500000	59.6
ccsA_p1	ccsA	1	TTCTCGATGGCTTCTCTTGC	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023566	126754	127719	1693-1693	0.500000	60.0
ccsA_p2	ccsA	2	TTCTCGATGGCTTCTCTTGC	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024937	126754	127719	1692-1692	0.500000	60.0
ccsA_p3	ccsA	3	CTCGATGGCTTCTCTTGCAT	GTGAAATCGGTAGACACGCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057002	126754	127719	1691-1691	0.500000	59.7
ccsA_p4	ccsA	4	CTCGATGGCTTCTCTTGCAT	TGAAATCGGTAGACACGCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058373	126754	127719	1690-1690	0.500000	59.7
ccsA_p5	ccsA	5	CGACATGGGACTCAATGGAG	GTGAAATCGGTAGACACGCT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.062443	126754	127719	1862-1862	0.500000	59.7
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	AGATTGGCCGAAAATCCCAT	20	20	50.0	45.0	58.0	57.5	0.0	10.0	0.467386	127877	128661	1514-1533	0.500000	57.1
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	AGATTGGCCGAAAATCCCAT	20	20	50.0	45.0	58.0	57.5	0.0	10.0	0.468756	127877	128661	1515-1534	0.500000	57.1
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	ATCCGCATAAAAGAGCTGCA	AGATTGGCCGAAAATCCCAT	20	20	45.0	45.0	58.0	57.5	1.0	10.0	0.502241	127877	128661	2102-2121	0.500000	56.6
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	TCCGCATAAAAGAGCTGCAT	AGATTGGCCGAAAATCCCAT	20	20	45.0	45.0	58.0	57.5	1.0	10.0	0.502241	127877	128661	2101-2120	0.500000	56.6
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AGCGTGTCTACCGATTTCAC	ACAGATTGGCCGAAAATCCC	20	20	50.0	50.0	58.0	58.5	0.0	10.0	0.533501	127877	128661	1516-1535	0.500000	56.6
rpl32-ndhF_p1	rpl32-ndhF	1	CAGTTCTTCGGGCTATGTCA	AATCGGTCGTGTGAACCAAA	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.716665	128836	129190	1064-1083	0.500000	55.1
rpl32-ndhF_p2	rpl32-ndhF	2	CAGTTCTTCGGGCTATGTCA	ATCGGTCGTGTGAACCAAAA	20	20	50.0	45.0	57.3	58.0	0.0	0.0	0.716665	128836	129190	1063-1082	0.500000	55.1
rpl32-ndhF_p3	rpl32-ndhF	3	CAGTTCTTCGGGCTATGTCA	CATACGTGGAGGGGGAATTG	20	20	50.0	55.0	57.3	58.0	0.0	0.0	0.705643	128836	129190	1300-1319	0.500000	55.0
rpl32-ndhF_p4	rpl32-ndhF	4	CAGTTCTTCGGGCTATGTCA	AGCAACTGCACCGACAAATA	20	20	50.0	45.0	57.3	58.1	0.0	4.2	0.782085	128836	129190	1271-1290	0.500000	54.4
rpl32-ndhF_p5	rpl32-ndhF	5	CAGTTCTTCGGGCTATGTCA	TAGCAACTGCACCGACAAAT	20	20	50.0	45.0	57.3	58.1	0.0	4.2	0.782085	128836	129190	1272-1291	0.500000	54.4
ndhF_p1	ndhF	1	CAGTTCTTCGGGCTATGTCA	CGGTTTGAAAATGCTGTACGA	20	21	50.0	42.9	57.3	57.5	0.0	0.0	2.202596	129191	131428	2939-2967	0.500000	42.9
ndhF_p2	ndhF	2	CAGTTCTTCGGGCTATGTCA	TCGGTTTGAAAATGCTGTACG	20	21	50.0	42.9	57.3	57.5	0.0	0.0	2.202596	129191	131428	2940-2968	0.500000	42.9
ndhF_p3	ndhF	3	TCAGTTCTTCGGGCTATGTC	CGGTTTGAAAATGCTGTACGA	20	21	50.0	42.9	57.0	57.5	0.0	0.0	2.491161	129191	131428	2940-2968	0.500000	40.6
ndhF_p4	ndhF	4	TCAGTTCTTCGGGCTATGTC	TCGGTTTGAAAATGCTGTACG	20	21	50.0	42.9	57.0	57.5	0.0	0.0	2.491161	129191	131428	2941-2969	0.500000	40.6
ndhF_p5	ndhF	5	CAGTTCTTCGGGCTATGTCAA	CGGTTTGAAAATGCTGTACGA	21	21	47.6	42.9	58.0	57.5	0.0	0.0	2.557009	129191	131428	2939-2967	0.500000	40.1