Hotspot

ndhF

Genome · 102486-104696 · 2211 bp

Back to Brachypodium

Pi 0.0155

MarkerSeek score 52.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2211
variable_sites: 51
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	TCCCCCATATAAGACGAGGG	45.0	55.0	58.0	57.7	2804-2824 bp	0.666667	66.9
ndhF_p2	ACCCATCAATGCATGGTGTT	TCCCCCATATAAGACGAGGG	45.0	55.0	58.1	57.7	2905-2925 bp	0.666667	66.7
ndhF_p3	AAAATCGCGGAAAACCGAAC	TAAGACGAGGGGGCAAAAAG	45.0	50.0	58.0	57.8	2815 bp	0.333333	51.5
ndhF_p4	AACACCCATCAATGCATGGT	TAAGACGAGGGGGCAAAAAG	45.0	50.0	58.1	57.8	2919 bp	0.333333	51.3
ndhF_p5	ACCCATCAATGCATGGTGTT	TAAGACGAGGGGGCAAAAAG	45.0	50.0	58.1	57.8	2916 bp	0.333333	51.3