Hotspot

ndhA

Genome · 112014-114149 · 2136 bp

Back to Brachypodium

Pi 0.0125

MarkerSeek score 51.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 2136
variable_sites: 40
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CAGCTTGTCGGACAGTACTC	ATGGGGGAAGTTGATCGTTG	55.0	50.0	58.0	57.9	2599-2604 bp	0.666667	67.0
ndhA_p2	CAGCTTGTCGGACAGTACTC	ATGATGGCCTTTTTCCCTGG	55.0	50.0	58.0	58.1	2730-2735 bp	0.666667	67.0
ndhA_p3	CAGCTTGTCGGACAGTACTC	GGAAAATTCGTCCACCTGGT	55.0	50.0	58.0	58.1	2711 bp	0.333333	52.5
ndhA_p4	CAGCTTGTCGGACAGTACTC	TGGAAAATTCGTCCACCTGG	55.0	50.0	58.0	58.1	2712 bp	0.333333	52.4
ndhA_p5	CTCCAATCCACAATGGGGAG	GGAAAATTCGTCCACCTGGT	55.0	50.0	57.9	58.1	2609 bp	0.333333	52.1