Hotspot

psbT-psbN

Genome · 70974-71021 · 48 bp

Back to Brachypodium

Pi 0.0833

MarkerSeek score 86.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: Genome
length_bp: 48
variable_sites: 6
pi: 0.0833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACCGCCTAAGGTTCCAACTA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.4	58.0	108 bp	1.0	81.4
psbT-psbN_p2	ACCGCCTAAGGTTCCAACTA	TCGCCATCTCCATATCTGGT	50.0	50.0	58.4	58.0	195 bp	1.0	79.5
psbT-psbN_p3	ACCGCCTAAGGTTCCAACTA	TCGAAGAACACGGGGACTAA	50.0	50.0	58.4	58.4	102 bp	1.0	78.8
psbT-psbN_p4	ACCGCCTAAGGTTCCAACTA	TTCGAAGAACACGGGGACTA	50.0	50.0	58.4	58.4	103 bp	1.0	78.8
psbT-psbN_p5	ACCGCCTAAGGTTCCAACTA	GTCGCCATCTCCATATCTGG	50.0	55.0	58.4	57.6	196 bp	1.0	76.6