Hotspot

petA-psbJ

Genome · 60936-61775 · 840 bp

Back to Brachypodium

Pi 0.0199

MarkerSeek score 66.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 840
variable_sites: 25
pi: 0.0199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	CTTTGGCTGATAGGTACGGT	50.0	50.0	57.9	57.0	1033-1038 bp	1.0	74.0
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGCTGATAGGTACGGTAACT	50.0	47.6	57.9	58.0	1030-1035 bp	1.0	73.7
petA-psbJ_p3	TGTTCTTCTTCGCATCCGTT	CTTTGGCTGATAGGTACGGT	45.0	50.0	57.8	57.0	1026-1031 bp	1.0	73.2
petA-psbJ_p4	TTGTTCTTCTTCGCATCCGT	CTTTGGCTGATAGGTACGGT	45.0	50.0	57.8	57.0	1027-1032 bp	1.0	73.2
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CTCTTTGGCTGATAGGTACGG	50.0	52.4	57.9	57.9	1035-1040 bp	1.0	73.1