Hotspot

ycf4-cemA

Genome · 58618-59046 · 429 bp

Back to Brachypodium

Pi 0.0205

MarkerSeek score 64.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 429
variable_sites: 13
pi: 0.0205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.9	57.3	512-519 bp	1.0	77.6
ycf4-cemA_p2	TGCGCGTACCAATTGAAGTA	TAACTGAGAAAGAGGCCCCC	45.0	55.0	57.9	58.7	530-537 bp	1.0	77.4
ycf4-cemA_p3	CTTGCGCGTACCAATTGAAG	CCCAGGGCAAAAGTACGATA	50.0	50.0	58.1	57.3	514-521 bp	1.0	77.3
ycf4-cemA_p4	CTTGCGCGTACCAATTGAAG	TAACTGAGAAAGAGGCCCCC	50.0	55.0	58.1	58.7	532-539 bp	1.0	77.1
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.8	57.3	541-548 bp	1.0	76.8