Hotspot

rbcL-rpl23

Genome · 56012-56336 · 325 bp

Back to Brachypodium

Pi 0.0168

MarkerSeek score 51.70

Primer available yes

Label rbcL-rpl23

Feature Metadata

label_name: rbcL-rpl23
feature_type: igs
region: Genome
length_bp: 325
variable_sites: 7
pi: 0.0168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-rpl23_p1	GATCATATCCACGCCGGTAC	GACTCACGATTACGCCTGTT	55.0	50.0	58.0	58.0	1122-1149 bp	1.0	82.1
rbcL-rpl23_p2	GATCATATCCACGCCGGTAC	GTTGACTCACGATTACGCCT	55.0	50.0	58.0	58.0	1125-1152 bp	1.0	82.1
rbcL-rpl23_p3	GATCATATCCACGCCGGTAC	ATCTCTACGGTGGATCCAGG	55.0	55.0	58.0	58.0	1303-1330 bp	1.0	82.0
rbcL-rpl23_p4	GATCATATCCACGCCGGTAC	TTGACTCACGATTACGCCTG	55.0	50.0	58.0	58.0	1124-1151 bp	1.0	82.0
rbcL-rpl23_p5	ATATCCACGCCGGTACAGTA	ATCTCTACGGTGGATCCAGG	50.0	55.0	58.0	58.0	1299-1326 bp	1.0	82.0