Hotspot

ndhC-trnV

Genome · 49946-50844 · 899 bp

Back to Brachypodium

Pi 0.0155

MarkerSeek score 66.10

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: Genome
length_bp: 899
variable_sites: 20
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	1001-1009 bp	1.0	82.0
ndhC-trnV_p2	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.0	58.3	1002-1010 bp	1.0	80.2
ndhC-trnV_p3	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	1024-1032 bp	1.0	78.5
ndhC-trnV_p4	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.4	57.9	1028-1036 bp	1.0	77.4
ndhC-trnV_p5	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.5	58.3	1025-1033 bp	1.0	76.6