Hotspot

trnL-trnF

Genome · 47221-47584 · 364 bp

Back to Brachypodium

Pi 0.0256

MarkerSeek score 53.20

Primer available yes

Label trnL-trnF

Feature Metadata

label_name: trnL-trnF
feature_type: igs
region: Genome
length_bp: 364
variable_sites: 13
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-trnF_p1	GTTGTGAGGGTTCAAGTCCC	TTTGAACTGGTGACACGAGG	55.0	50.0	58.4	58.1	427-453 bp	1.0	82.8
trnL-trnF_p2	GTTGTGAGGGTTCAAGTCCC	CTGCCAGGAACCAGATTTGA	55.0	50.0	58.4	57.8	442-468 bp	1.0	81.5
trnL-trnF_p3	GTTGTGAGGGTTCAAGTCCC	TCTGCCAGGAACCAGATTTG	55.0	50.0	58.4	57.8	443-469 bp	1.0	81.5
trnL-trnF_p4	GTTGTGAGGGTTCAAGTCCC	TTTTCTCTGCCAGGAACCAG	55.0	50.0	58.4	57.7	448-474 bp	1.0	80.9
trnL-trnF_p5	AGTTGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	428-454 bp	1.0	80.8