Hotspot

petN-trnC

Genome · 17574-18508 · 935 bp

Back to Brachypodium

Pi 0.0146

MarkerSeek score 63.10

Primer available yes

Label petN-trnC

Feature Metadata

label_name: petN-trnC
feature_type: igs
region: Genome
length_bp: 935
variable_sites: 20
pi: 0.0146
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC_p1	TTAAAGCAGCCCAAGCAAGA	ACGTGGGTGAATACTTGCTC	45.0	50.0	58.0	57.9	1087-1088 bp	1.0	80.7
petN-trnC_p2	CAGCCCAAGCAAGACTTACT	ACGTGGGTGAATACTTGCTC	50.0	50.0	57.8	57.9	1081-1082 bp	1.0	79.2
petN-trnC_p3	TTAAAGCAGCCCAAGCAAGA	CGTGGGTGAATACTTGCTCT	45.0	50.0	58.0	57.6	1086-1087 bp	1.0	78.3
petN-trnC_p4	TTAAAGCAGCCCAAGCAAGA	AACGTGGGTGAATACTTGCT	45.0	45.0	58.0	57.4	1088-1089 bp	1.0	76.9
petN-trnC_p5	CAGCCCAAGCAAGACTTACT	CGTGGGTGAATACTTGCTCT	50.0	50.0	57.8	57.6	1080-1081 bp	1.0	76.9