Hotspot

rps16-trnQ

Genome · 5597-6226 · 630 bp

Back to Brachypodium

Pi 0.0149

MarkerSeek score 61.50

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: Genome
length_bp: 630
variable_sites: 14
pi: 0.0149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	703-839 bp	1.0	80.0
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	714-850 bp	1.0	79.5
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	45.0	45.0	58.1	57.8	772-908 bp	1.0	79.1
rps16-trnQ_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	707-843 bp	1.0	78.9
rps16-trnQ_p5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.2	57.8	783-919 bp	1.0	78.5