Markers + reference

Brachyglottis

2 species · Asteraceae · Asterales

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Species 2
Genome length 151–151 kb
Candidate markers 267
Primer pairs

Genome-wide nucleotide diversity

Sliding-window nucleotide diversity
Sliding-window nucleotide diversity (π) across the plastome.

Candidate markers

9 hotspot labels from the diversity plot in genomic order, plus the top 9 remaining regions by MarkerSeek score (out of 267 candidates).

Label Region Length (bp) Pi Alignment reliability MarkerSeek score Primer available Action
trnK-UUU-rps16 LSC 811 0.0088 0.98 50.4 no View details
rps16-trnQ-UUG LSC 962 0.0084 0.99 62.7 no View details
trnC-GCA-petN LSC 863 0.0092 0.76 59.9 no View details
rpoC2 LSC 4152 0.0022 1.00 47.2 no View details
pafI-trnS-GGA LSC 872 0.0069 1.00 58.2 no View details
trnT-UGU-trnL-UAA LSC 609 0.0072 0.92 59.9 no View details
petD LSC 1201 0.0042 1.00 61.1 no View details
trnL-UAG-rpl32 SSC 338 0.0137 0.87 60.7 no View details
rpl32-ndhF SSC 1054 0.0058 0.99 61.4 no View details
petA-psbJ LSC 756 0.0053 1.00 61.8 no View details
ndhK LSC 678 0.0044 1.00 61.3 no View details
psbE-petL LSC 1229 0.0025 0.99 60.7 no View details
accD-psaI LSC 659 0.0030 1.00 60.5 no View details
petB LSC 1405 0.0028 1.00 60.4 no View details
psaA-pafI LSC 695 0.0029 1.00 60.3 no View details
rpoA LSC 1008 0.0020 1.00 59.9 no View details
psbD LSC 1062 0.0019 1.00 59.8 no View details
atpF LSC 1248 0.0016 1.00 59.7 no View details
Candidate markers include all hotspot labels marked in the diversity plot, listed in genomic order, plus the top 10 remaining regions by MarkerSeek score. Primer design is attempted for every listed region. Primer available: yes means at least one valid primer pair was found; no means no valid pair passed screening; NA means primer design was skipped.

Result downloads

Reference species (2)

One GenBank record per species. Use the NCBI button to open the Nucleotide entry for the listed accession.

Species Accession Length (bp) Link
Brachyglottis kirkii PX652375.1 150575 View on NCBI ↗
Brachyglottis perdicioides PQ385615.1 150611 View on NCBI ↗