Hotspot

rpl16

LSC · 80029-81602 · 1574 bp

Back to Brachycorythis

Pi 0.0032

MarkerSeek score 52.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1574
variable_sites: 5
pi: 0.0032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AGAGATAAAGAAACCCGCGT	AGAACAATTCTAATTCTATAAGGTTGA	45.0	25.9	56.9	54.9	1693-1705 bp	1.0	40.8
rpl16_p2	TCCAGAGATAAAGAAACCCGC	AGAACAATTCTAATTCTATAAGGTTGA	47.6	25.9	57.7	54.9	1696-1708 bp	1.0	40.8
rpl16_p3	AGAGATAAAGAAACCCGCGT	GAACAATTCTAATTCTATAAGGTTGA	45.0	26.9	56.9	53.7	1692-1704 bp	1.0	40.8
rpl16_p4	AGAGATAAAGAAACCCGCGTT	AGAACAATTCTAATTCTATAAGGTTGA	42.9	25.9	57.7	54.9	1693-1705 bp	1.0	40.8
rpl16_p5	AGAGATAAAGAAACCCGCGT	AGAACAATTCTAATTCTATAAGGTTG	45.0	26.9	56.9	53.7	1693-1705 bp	1.0	40.8