Hotspot

clpP-psbB

LSC · 70256-70906 · 651 bp

Back to Brachycorythis

Pi 0.00461

MarkerSeek score 61.40

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 3
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CCAGCCCAACCAGAAACTAG	50.0	55.0	58.0	58.2	831 bp	1.0	78.9
clpP-psbB_p2	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	795 bp	1.0	78.8
clpP-psbB_p3	CAAACTGCATCCTCCTCTCC	CCAGCCCAACCAGAAACTAG	55.0	55.0	58.0	58.2	814 bp	1.0	78.6
clpP-psbB_p4	CCAAACTGCATCCTCCTCTC	CCAGCCCAACCAGAAACTAG	55.0	55.0	58.0	58.2	815 bp	1.0	78.6
clpP-psbB_p5	CAAACTGCATCCTCCTCTCC	TGGACAGAAAGCAATCGACC	55.0	50.0	58.0	58.2	778 bp	1.0	78.5