Hotspot

petA-psbJ

LSC · 61808-62606 · 799 bp

Back to Brachycorythis

Pi 0.00501

MarkerSeek score 48.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 4
pi: 0.00501
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTTCAGTTGTACGAAATGAATTTCT	TATTCCGGGTTGGGTTCATC	32.0	50.0	56.6	57.3	853 bp	1.0	40.4
petA-psbJ_p2	GTTCAGTTGTACGAAATGAATTTCT	CTATTCCGGGTTGGGTTCAT	32.0	50.0	56.6	57.3	854 bp	1.0	40.4
petA-psbJ_p3	TCAGTTGTACGAAATGAATTTCT	TATTCCGGGTTGGGTTCATC	30.4	50.0	54.6	57.3	851 bp	1.0	40.4
petA-psbJ_p4	TCAGTTGTACGAAATGAATTTCT	CTATTCCGGGTTGGGTTCAT	30.4	50.0	54.6	57.3	852 bp	1.0	40.4
petA-psbJ_p5	GTTCAGTTGTACGAAATGAAT	TATTCCGGGTTGGGTTCATC	33.3	50.0	52.5	57.3	853 bp	1.0	40.4