Hotspot

ndhC-trnV-UAC

LSC · 49788-50624 · 837 bp

Back to Brachycorythis

Pi 0
MarkerSeek score 60.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
837
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCTTCCCTAATCGGAGCTA CCGAGAAAGTCTACGGTTCG 55.0 55.0 58.0 58.1 968-984 bp 1.0 80.4
ndhC-trnV-UAC_p2 CCCTTCCCTAATCGGAGCTA GTTCGAGTCCGTATAGCCCT 55.0 55.0 58.0 58.7 953-969 bp 1.0 75.9
ndhC-trnV-UAC_p3 CCCTTCCCTAATCGGAGCTA CGAGAAAGTCTACGGTTCGA 55.0 50.0 58.0 57.1 967-983 bp 1.0 74.2
ndhC-trnV-UAC_p4 CCCTTCCCTAATCGGAGCTA TTCGAGTCCGTATAGCCCTA 55.0 50.0 58.0 57.1 952-968 bp 1.0 73.8
ndhC-trnV-UAC_p5 CTTTTCTGGCCCTTCCCTAA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.1 58.1 977-993 bp 1.0 73.3