Hotspot

psaA-ycf3

LSC · 41067-41695 · 629 bp

Back to Brachycorythis

Pi 0.00161

MarkerSeek score 60.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 1
pi: 0.00161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GGACAGGCTTGGTTTGATCA	50.0	50.0	57.9	58.1	814-822 bp	1.0	79.4
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TGGACAGGCTTGGTTTGATC	50.0	50.0	57.9	58.1	815-823 bp	1.0	79.4
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	769-777 bp	1.0	78.5
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	768-776 bp	1.0	78.5
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	805-813 bp	1.0	77.9